Base de dados : HANSEN
Pesquisa : ESTUDOS TRANSVERSAIS [Descritor de assunto]
Referências encontradas : 12 [refinar]
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Id:23189
Autor:Young, Saroj K; Taylor, G. Michael; Jain, Suman; Suneetha, Lavanya M; Suneetha, Sujai; Lockwood, Diana N. J; Young, Douglas B
Título:Microsatellite mapping of Mycobacterium leprae populations in infected humans
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Fonte:s.l; s.n; Nov. 2004. 6 p. ilus, mapas, tab.
Resumo:To investigate genetic diversity in a bacterial population, we measured the copy numbers of simple sequence repeats, or microsatellites, in Mycobacterium leprae from patients living in and around Hyderabad, India. Three microsatellite loci containing trinucleotide or dinucleotide repeats were amplified from infected tissues, and the copy numbers were established by sequence analysis. Extensive diversity was observed in a cross-sectional survey of 33 patients, but closely related profiles were found for members of a multicase family likely to share a common transmission source. Sampling of multiple tissues from single individuals demonstrated identical microsatellite profiles in the skin, nasal cavity, and bloodstream but revealed differences at one or more loci for M. leprae present in nerves. Microsatellite mapping of M. leprae represents a useful tool for tracking short transmission chains. Comparison of skin and nerve lesions suggests that the evolution of disease within an individual involves the expansion of multiple distinct subpopulations of M. leprae. (AU).
Descritores:Técnica de Tipagem Bacteriana/*
Estudos Transversais
Família
Dosagem de Genes
Hanseníase/EP/*MI/*TM
Repetições de Microssatélites/*GE
Mycobacterium leprae/*CL/*GE
Reação em Cadeia da Polimerase
Especificidade de Espécies
Variação (Genética)/*
Limites:HUMANO
MASCULINO
FEMININO
SUPPORT, NON-U.S. GOV'T
Localização:BR191.1; 09333/s


  2 / 12 HANSEN  
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Id:18491
Autor:Guerrero, Martha Inírida; Arias, María Teresa; Garcés, María Teresa; León, Clara Inés
Título:Desarrollo y aplicación de una prueba de RCP para detectar la infección subclínica por Mycobacterium leprae
Developing and using a PCR test to detect subclinical Mycobacterium leprae infection-
Fonte:s.l; s.n; 2002. 7 p. ilus, tab.
Resumo:OBJECTIVE: While the prevalence of leprosy has declined around the world, there has not been a corresponding decrease in its incidence, thus indicating that it has not been possible to prevent transmission of the disease. Despite the small number of patients with lepromatous leprosy, the majority of the inhabitants of endemic areas show signs of exposure to Mycobacterium leprae, which could be explained by the presence of subclinical bacilliferous infections in the community. The objective of this study was to investigate the use of a polymerase chain reaction (PCR) test to detect M. leprae in samples of nasal mucus from asymptomatic household contacts of patients with leprosy. METHODS: We standardized and optimized a PCR technique to amplify a 321 base pair DNA fragment, using a pair of primers complementary to a segment of an LSR/A15 gene that codes for the 15 kDa M. leprae antigen. We investigated the optimal concentrations of all the test components. We used dimethyl sulfoxide (DMSO) to achieve a more specific amplification. We applied the PCR test to 70 healthy household contacts of leprosy patients from eight municipalities in Colombia where there was a high prevalence of the disease. RESULTS: The test's detection limit was 100 fg of DNA. With the optimized technique, bacillus was detected in the nasal mucus samples of 9 (12.8 per cent) of the 70 household contacts. The 3 PCR-positive household contacts of paucibacillary cases were from municipalities with very high prevalence levels. In comparison to contacts who were PCR-negative, the contacts who were PCR-positive had spent significantly less time, as a proportion of their age, living with a patient (P = 0.028). This finding demonstrates the test's capacity for early detection. CONCLUSIONS: The PCR test that we developed is useful as a tool for detection and early follow-up of possible leprosy cases. It can be used to monitor high-risk populations and also to maintain the achievements of leprosy elimination programs in countries where the disease's prevalence has been significantly reduced. (AU).
Descritores:ESTUDOS TRANSVERSAIS
HANSENIASE/diag
HANSENIASE/epidemiol
MYCOBACTERIUM LEPRAE
REACAO EM CADEIA DA POLIMERASE/métodos
Limites:HUMANO
MASCULINO
FEMININO
ADULTO
SUPPORT, NON-U.S. GOV'T
RESUMO EM INGLES
Localização:BR191.1; 09066/s


  3 / 12 HANSEN  
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Id:16332
Autor:Keita, S; Tiendrebeogo, A; Konare, H; Cisse, B.-S; Faye, O
Título:Nouveaux cas de lèpre à L'Institut Marchoux (Bamako, Mali): Etude comparative 1988-1997
New cases of leprosy at the Marchoux Institute: a comparative study 1988-1997-
Fonte:s.l; s.n; 2001. 3 p. tab.
Descritores:CRIANÇA
ESTUDOS TRANSVERSAIS
HANSENIASE
MALI
ESTUDOS RETROSPECTIVOS
ESTUDOS PROSPECTIVOS
Limites:ESTUDO COMPARATIVO
Localização:BR191.1; 08641/s


  4 / 12 HANSEN  
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Id:15711
Autor:Prasad, P. V. S
Título:Childhood leprosy in a rural hospital
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Fonte:s.l; s.n; 1998. 4 p. tab.
Descritores:ADOLESCENCIA
CRIANÇA
PRÉ-ESCOLAR
ESTUDOS TRANSVERSAIS
HOSPITAIS RURAIS
INCIDENCIA
INDIA
LACTENTE
HANSENIASE
HANSENIASE
POPULAÇAO RURAL
Localização:BR191.1; 08538/s


  5 / 12 HANSEN  
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Id:14350
Autor:Anon
Título:Progress towards the elimination of leprosy as a public health problem
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Fonte:s.l; s.n; 1995. 6 p. tab.
Descritores:COMPARAÇAO TRANSCULTURAL
ESTUDOS TRANSVERSAIS
PREVISOES
INCIDENCIA
HANSENIASE
HANSENIASE
SAUDE PUBLICA
ORGANIZAÇAO MUNDIAL DA SAUDE
Limites:ESTUDO COMPARATIVO
Localização:BR191.1; 07200/s


  6 / 12 HANSEN  
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Id:14348
Autor:Anon
Título:Leprosy disabilities: magnitude of the problem
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Fonte:s.l; s.n; 1995. 7 p. tab, graf.
Descritores:ADOLESCENCIA
CRIANÇA
PRÉ-ESCOLAR
ESTUDOS TRANSVERSAIS
PAISES EM DESENVOLVIMENTO
AVALIAÇAO DA INCAPACIDADE
DEFICIENTES
QUIMIOTERAPIA COMBINADA
INCIDENCIA
LACTENTE
LEPROSTATICOS
LEPROSTATICOS
HANSENIASE
HANSENIASE
ESPERANÇA DE VIDA
ORGANIZAÇAO MUNDIAL DA SAUDE
Localização:BR191.1; 07197/s


  7 / 12 HANSEN  
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Id:13976
Autor:Ulrich, Marian; Zulueta, Ana Maria; Caceres-Dittmar, Gisela; Sampson, Celsa; Pinardi, Maria Eugenia; Rada, Elsa Maria; Aranzazu, Nacarid
Título:Leprosy in women: characteristics and repercussions
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Fonte:s.l; s.n; 1993. 12 p. tab, graf.
Descritores:ESTUDOS TRANSVERSAIS
PAISES EM DESENVOLVIMENTO
AVALIAÇAO DA INCAPACIDADE
EDUCAÇAO EM SAUDE
INCIDENCIA
HANSENIASE
HANSENIASE
HANSENIASE
HANSENIASE
ACEITAÇAO SOCIAL
FATORES SOCIO-ECONOMICOS
Localização:BR191.1; 06622/s


  8 / 12 HANSEN  
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Id:13967
Autor:Anon
Título:Progress towards the elimination of leprosy as a public health problem
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Fonte:s.l; s.n; 1993. 6 p. ilus, tab, mapas.
Descritores:CONTROLE DE DOENÇAS TRANSMISSIVEIS
COMPARAÇAO TRANSCULTURAL
ESTUDOS TRANSVERSAIS
PAISES EM DESENVOLVIMENTO
QUIMIOTERAPIA COMBINADA
INCIDENCIA
LEPROSTATICOS
HANSENIASE
HANSENIASE
ORGANIZAÇAO MUNDIAL DA SAUDE
Localização:BR191.1; 06613/s


  9 / 12 HANSEN  
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Id:13900
Autor:Frommel, Dominique; Tekle-Haimanot, Redda; Verdier, Mireille; Negesse, Yohannes; Bulto, Tesfaye; Denis, Francois
Título:HIV infection and leprosy: a four-year survey in Ethiopia
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Fonte:s.l; s.n; 1994. 2 p. tab.
Descritores:ESTUDOS TRANSVERSAIS
ETIOPIA
INFECÇOES POR HIV
INFECÇOES POR HIV
HIV-1
HANSENIASE
HANSENIASE
MORBIDADE
Localização:BR191.1; 06889/s


  10 / 12 HANSEN  
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Id:13685
Autor:Frommel, Dominique; Tekle-Haimanot, Redda; Berhe, Nega; Aussel, Laure; Verdier, Mireille; Preux, Pierre-Marie; Denis, Francois
Título:A survey of antibodies to hepatitis C virus in Ethiopia
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Fonte:s.l; s.n; 1993. 5 p. tab.
Descritores:ADOLESCENCIA
FATORES ETARIOS
CRIANÇA
ESTUDOS DE COORTES
ESTUDOS TRANSVERSAIS
ETIOPIA
ANTICORPOS ANTI-HEPATITE
HEPATITE C
HEPATITE C
ANTICORPOS PRESENTES EM HEPATITE C
TÉCNICAS IMUNOENZIMATICAS
HANSENIASE
DOENÇAS DO SISTEMA NERVOSO
PREVALENCIA
FATORES DE RISCO
POPULAÇAO RURAL
DERMATOPATIAS
HEPACIVIRUS/*IM
DERMATOPATIAS/CO
Localização:BR191.1; 06663/s


  11 / 12 HANSEN  
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Id:13316
Autor:Kumar, Dinesh B; Prasad, Eshwar C; Krishnaswamy, Kamala
Título:Detection of rifampicin-induced nephrotoxicity by N-acetyl-3-D-glucosaminidase activity
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Fonte:s.l; s.n; 1992. 4 p. graf.
Descritores:HANSENIASE
ANTIBIOTICOS COMBINADOS
ANTIBIOTICOS COMBINADOS
ESTUDOS TRANSVERSAIS
SINERGISMO DE DROGAS
TAXA DE FILTRAÇAO GLOMERULAR
RIM
RIFAMPINA
RIFAMPINA
ESTREPTOMICINA
ESTREPTOMICINA
TUBERCULOSE PULMONAR
Limites:HUMANO
MEIA-IDADE
Localização:BR191.1; 06474/s


  12 / 12 HANSEN  
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Id:5485
Autor:Pfau, Ruth
Título:Analysis of leprosy control programme in Pakistan
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Fonte:s.l; s.n; 1984. 13 p. tab.
Descritores:HANSENIASE
TERAPIA COMBINADA
ESTUDOS TRANSVERSAIS
Limites:HUMANO
Localização:BR191.1; 02449/s



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